Monoclonal IgG to the inner capsid of human rotavirus
نویسندگان
چکیده
منابع مشابه
Serum antibody response to recombinant major inner capsid protein following human infection with group B rotavirus.
Recombinant major inner capsid protein (VP6) of the IDIR strain of group B rotavirus (GBR) was incorporated in a solid-phase immunoassay to access antibody response to infection in humans. Expression of VP6 in insect cells permitted design of a highly sensitive assay that avoided the contaminants present in GBR antigens obtained from fecal specimens. Among patients infected with the ADRV strain...
متن کاملThe structure of the rotavirus inner capsid studied by electron microscopy of chemically disrupted particles.
The inner capsid structure of the OSU strain of porcine rotavirus was studied by electron microscopy of freeze-dried preparations and of negatively stained chemically disrupted virus particles. The analysis of the particles by the freeze-drying technique revealed a T:13 l (laevo) symmetry for the organization of the inner capsid. Treatment of single-capsid rotavirus particles with 30% formamide...
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Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...
متن کاملHeterologous protection induced by the inner capsid proteins of rotavirus requires transcytosis of mucosal immunoglobulins.
Protective immunization against rotavirus (RV) can be achieved with heterologous RV, i.e., virus isolated from another species, and with heterologous inner core VP2 and VP6 proteins assembled as virus-like particles (VLP). Although the antigenically conserved VP6 protein does not induce in vitro-neutralizing antibodies, it may, however, elicit immunoglobulins (Ig) involved in heterologous prote...
متن کاملX-ray crystal structure of the rotavirus inner capsid particle at 3.8 A resolution.
The rotavirus inner capsid particle, known as the "double-layered particle" (DLP), is the "payload" delivered into a cell in the process of viral infection. Its inner and outer protein layers, composed of viral protein (VP) 2 and VP6, respectively, package the 11 segments of the double-stranded RNA (dsRNA) of the viral genome, as well as about the same number of polymerase molecules (VP1) and c...
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ژورنال
عنوان ژورنال: Archives of Virology
سال: 1982
ISSN: 0304-8608,1432-8798
DOI: 10.1007/bf01314729